کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8476771 | 1550851 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of glucocorticoids on the activity, expression and proximal promoter of type II deiodinase in rat brown adipocytes
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کلمات کلیدی
TCARIAActDGRECHXGATA4PTUBATNCSTTF1qRT-PCRDIO1NF-kB6-propyl-2-thiouracilDTTHEPESUBCCREDEXCCAAT enhancer binding proteinsDMEMLPSBr-cAMP4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid - 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acidcyclic AMP - AMP cyclicC/EBP - C / EBPcAMP - cAMPDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoUcp1 - UCP1Brown adipocytes - آدفوسیت های قهوه ایtrichloroacetic acid - اسید ترشکلراکتیکactinomycin D - اکتینومایسین Dbrown adipose tissue - بافت چربی قهوه ایTriiodothyronine - تری یدوتیرونینthyroxine - تیروکسینDexamethasone - دگزامتازونDio3 - دیو 3dithiothreitol - دیتیوتریتولradioimmunoassay - رادیوایمونواسیnewborn calf serum - سرم گاو تازه متولد شدهcycloheximide - سیکلوهایسیمیدthyroid transcription factor - عامل رونویسی تیروئیدcAMP response element - عنصر پاسخ cAMPGlucocorticoid response element - عنصر پاسخ گلوکوکورتیکوئیدnuclear factor kappa beta - فاکتور هسته ای کاپا بتاlipopolysaccharide - لیپوپلی ساکاریدnorepinephrine - نوراپی نفرینtype 2 deiodinase - نوع 2 دئودینازtype 3 deiodinase - نوع 3 دئودینازHydrocortisone - هیدروکورتیزونquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیuncoupling protein 1 - پروتئین جدا سازی 1glucocorticoid receptor - گیرنده گلوکوکورتیکوئیدUbiquitin - یوبیکویتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effect of glucocorticoids on the activity, expression and proximal promoter of type II deiodinase in rat brown adipocytes Effect of glucocorticoids on the activity, expression and proximal promoter of type II deiodinase in rat brown adipocytes](/preview/png/8476771.png)
چکیده انگلیسی
Triiodothyronine (T3) is important for thermogenesis in brown adipose tissue (BAT). Type II deiodinase (DIO2) produces T3 required for intracellular needs in BAT. Brown adipocytes in culture require T3 for the adrenergic stimulation of DIO2. Glucocorticoids induce adipocyte differentiation (lipogenesis). We investigated the regulation of DIO2 activity, Dio2 mRNA and Dio2 promoter activity by glucocorticoids in primary cultures of rat brown adipocytes using dexamethasone (DEX) and hydrocortisone (HC). DEX and HC regulated the adrenergic stimulation of DIO2 activity in a dose- and time-dependent manner, inhibiting DIO2 activity at short treatment times and large doses (1-10 μM) and stimulating DIO2 at low HC doses (1-100 nM) and longer times (DEX). Insulin depletion reduced DIO2 activity but the response to glucocorticoids remained unchanged. DEX and HC inhibited basal DIO2 activity. DEX had no effect on DIO2 half-life, whereas HC stabilized DIO2 activity. DEX and HC inhibited the adrenergic stimulation of Dio2 mRNA expression (100-10000 nM, 14-96 h), but stabilized Dio2 mRNA, particularly DEX. DEX increased basal Dio2 mRNA levels, possibly through stabilization of Dio2 mRNA. An 807 bp construct of the murine Dio2 proximal promoter showed maximal reporter activity, with the cAMP response element (CRE) essential for transcriptional activity. DEX caused inhibition in most constructs containing the CRE element whereas HC stimulated reporter activity in the 807 bp construct. Glucocorticoids inhibited the adrenergic stimulation of Dio2 at the transcriptional level in brown adipocytes, although DIO2 activity increased with HC, possibly due to stabilization of Dio2 activity and mRNA. The CRE and cEBP elements of the Dio2 promoter seem involved in the regulation by glucocorticoids.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 428, 15 June 2016, Pages 58-67
Journal: Molecular and Cellular Endocrinology - Volume 428, 15 June 2016, Pages 58-67
نویسندگان
Raquel Martinez-deMena, Rosa-Maria Calvo, Laura Garcia, Maria Jesus Obregon,