کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8477279 | 1550896 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Use of detergent-based buffers allows detection of precursor inhibin forms in an immunoassay format
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Inhibin ELISAs are used in monitoring aspects of reproductive function, however these assays are based on the measurements of the mature 30 kDa inhibin forms and not precursor forms. In conventional ELISA formats, the 105 kDa unprocessed 'Pro-inhibin' forms are immunologically inactive, but the immunoactivity can be recovered in the presence of detergents. The immunoactivity of Pro-inhibin forms was assessed in the presence of a range of detergents utilising antibodies to the α-, βA- and βB-subunits of inhibin. In contrast to mature forms, unprocessed inhibin forms showed a 10-40 fold increase in inhibin A and total inhibin immunoactivities under optimised detergent (0.5% SDS/2% Triton X-100) conditions. The suppressed immunoactivity of the Pro-inhibin forms in these immunoassays was attributed to steric hindrance by the respective βA- and α-subunit prodomains. This study details a detergent-based immunoassay that allows detection of previously undetectable precursor inhibin forms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 381, Issues 1â2, 5 December 2013, Pages 106-114
Journal: Molecular and Cellular Endocrinology - Volume 381, Issues 1â2, 5 December 2013, Pages 106-114
نویسندگان
Kelly L. Walton, Karen L. Chan, Enid Pruysers, Emily K. Kelly, Guy Harris, Craig A. Harrison, David M. Robertson,