Correlation between secosteroid-induced vitamin D receptor activity in melanoma cells and computer-modeled receptor binding strength

► We tested ligand induced vitamin D receptor translocation by novel secosteroids. ► We modeled secosteroids bindings to the genomic and non-genomic pockets. ► Good correlation between binding to the genomic pocket and translocation exists. ► Novel vitamin D hydroxyderivatives inhibit cell proliferation. ► Both chain length and the number or positions of hydroxyl groups are important.