کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8479199 | 1551295 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
l-Carnitine attenuates H2O2-induced neuron apoptosis via inhibition of endoplasmic reticulum stress
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کلمات کلیدی
DCFDAJnkCCAAT/enhancer-binding protein-homologous proteinESRtunicamycinNACERK2′,7′-dichlorodihydrofluorescein diacetate - 2 '، 7'-dichlorodihydrofluorescein diacetatec-Jun N-terminal protein kinase - C-Jun N-terminal protein kinaseGRP78/BiP - GRP78 / BIPMAPK - MAPKN-acetylcysteine - N-استیل سیستئینROS - ROSHydrogen peroxide - آب اکسیژنهER stress - استرس استEndoplasmic reticulum stress - استرس شبکه آندوپلاسمیl-Carnitine - ال کارنیتینOxidative stress - تنش اکسیداتیوCHOP - تکه کردنApoptosis - خزان یاختهایSH-SY5Y cells - سلول های SH-SY5Yextracellular-signal regulated kinase - سیگنال تنظیم شده سیگنال خارج سلولیH2O2 - هیدروژن پراکسیدglucose-regulated protein 78 - پروتئین تنظیم شده با گلوکز 78mitogen-activated protein kinase - پروتئین کیناز فعال با mitogenReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Both oxidative stress and endoplasmic reticulum stress (ER stress) have been linked to pathogenesis of neurodegenerative diseases. Our previous study has shown that l-carnitine may function as an antioxidant to inhibit H2O2-induced oxidative stress in neuroblastoma SH-SY5Y cells. To further explore the neuroprotection of l-carnitine, here we study the effects of l-carnitine on the ER stress response in H2O2-induced SH-SY5Y cell injury. Our results showed that l-carnitine pretreatment could increase cell viability; inhibit apoptosis and ROS accumulation caused by H2O2 or tunicamycin (TM). l-carnitine suppress the endoplasmic reticulum dilation and activation of ER stress-associated proteins including glucose-regulated protein 78 (GRP78), CCAAT/enhancer-binding protein-homologous protein (CHOP), JNK, Bax and Bim induced by H2O2 or TM. In addition, H2O2-induced cell apoptosis and activation of ER stress can also be attenuated by antioxidant N-acetylcysteine (NAC), CHOP siRNA and the inhibitor of ER stress 4-phenylbutyric acid (4-PBA). Taken together, our results demonstrated that H2O2 could trigger both oxidative stress and ER stress in SH-SY5Y cells, and ER stress participated in SH-SY5Y apoptosis mediated by H2O2-induced oxidative stress. CHOP/Bim or JNK/Bim-dependent ER stress signaling pathways maybe related to the neuroprotective effects of l-carnitine against H2O2-induced apoptosis and oxidative injury.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurochemistry International - Volume 78, December 2014, Pages 86-95
Journal: Neurochemistry International - Volume 78, December 2014, Pages 86-95
نویسندگان
Junli Ye, Yantao Han, Xuehong Chen, Jing Xie, Xiaojin Liu, Shunhong Qiao, Chunbo Wang,