کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8481062 | 1551410 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Astrocyte elevated gene-1 promotes inflammation and invasion of fibroblast-like synoviocytes in rheumatoid arthritis
ترجمه فارسی عنوان
افزایش سطح هورمون استروسیتی ژن 1 باعث التهاب و حمله سینوویوسیت های فیبروبلاست مانند در آرتریت روماتوئید می شود
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کلمات کلیدی
NF-κBMMPPI3 KAEG-1IL-6FLSIL-1βIKKIκB kinase - IkB kinaseRheumatoid arthritis - آرتریتروماتوئیدAkt - آکتinflammation - التهاب( توروم) interleukin-6 - اینترلوکین ۶Interleukin-1β - اینترلوکین-1βInvasion - تهاجمtumor necrosis factor-α - تومور نکروز عامل αTNF-α - فاکتور نکروز توموری آلفاnuclear factor-κB - فاکتور هسته ای κBmatrix metalloproteinase - ماتریکس متالوپروتئینازfibroblast-like synoviocytes - مانند سینوویوسیت های فیبروبلاستprotein kinase B - پروتئین کیناز Bastrocyte elevated gene-1 - ژن 1 افزایش یافته است
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
Astrocyte elevated gene-1 (AEG-1) was initially induced by HIV-1 infection and involved in tumor progression, migration and invasion as a nuclear factor-κB (NF-κB)-dependent gene. The present study we intended to investigate the protein expression of AEG-1 significantly associated with rheumatoid arthritis. Western blot analysis and immunohistochemistry demonstrated that AEG-1 was upregulated in synovial tissue of RA patients compared with the controls. Double immunofluorescent staining suggested that AEG-1 was expressed in fibroblast-like synoviocytes (FLS) of RA patients. Furthermore, the expression of AEG-1 in FLS was increased in time-dependent manner by TNF-α stimulation. Upon TNF-α-treated FLS, AEG-1 transferred from the cytoplasm to nucleus where it interacted with the p65 subunit of NF-κB, as examined by immunoprecipitation and immunofluorescent staining assay. Moreover, the inhibition of AEG-1 by RNA interference significantly suppressed TNF-α-induced IL-6 and MMP-3 expression, leading to attenuation of FLS migration and invasion and markedly decreased the phosphorylation of P65 and IκBα, as well as AKT in FLS. Collectively, Our findings provided evidence that AEG-1 contributed to the production of inflammatory cytokines, migration and invasion of RA FLS, and underscored the importance of AEG-1 in the inflammation process of RA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tissue and Cell - Volume 49, Issue 6, December 2017, Pages 672-679
Journal: Tissue and Cell - Volume 49, Issue 6, December 2017, Pages 672-679
نویسندگان
Ruilong Hong, Kun Wang, Hongguang Shi,