کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8490890 | 1552342 | 2018 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A small scale rumen incubation system to screen chemical libraries for potential methane inhibitors
ترجمه فارسی عنوان
یک سیستم انکوباسیون شکمبه ای کوچک برای نمایش کتابخانه های شیمیایی برای مهار کننده های متان بالقوه
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کلمات کلیدی
DMFBESNOP3-Nitrooxypropanol - 3-نیتروکسی پروپانول9,10-Anthraquinone - 9،10 آنتراکینونDMSO - DMSOHigh-throughput - با توان بالاInhibitor - بازدارندهIn vitro - درونکشتگاهیdimethylformamide - دی متیل فرمالیدDimethyl sulfoxide - دیمتیل سولفواکسیدRumen - شکمبه، سیرابیMethane - متان gas chromatograph - کروماتوگرافی گاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
There is currently an urgent need for effective anti-methanogen small molecule inhibitors to help control ruminant methane emissions on a global scale. No assays system has yet been reported that would enable high-throughput screening of either rumen methanogen cultures or an assay system that utilises rumen fluid. We report here the development of a high-throughput 0.2âml rumen fluid-based 96-well screening system that incorporates a simple substrate for fermentation with a short incubation time The fermentation parameters determined are total gas, methane and hydrogen production. The system markedly enhances the number of samples that can be processed (â¼1000 incubation vials per week) compared to previously published assays. The system does not try to mimic rumen fermentation but is a means of screening compounds for their potential effect against rumen methanogens. We also assessed the system using known rumen anti-methanogen inhibitory compounds, a set of anthraquinone derivatives and 3-nitrooxypropanol. The system can differentiate between specific methane inhibition and the inhibition of overall fermentation and it discriminates between molecules that act as methanogen inhibitors and those acting as electron sinks.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Feed Science and Technology - Volume 244, October 2018, Pages 88-92
Journal: Animal Feed Science and Technology - Volume 244, October 2018, Pages 88-92
نویسندگان
Stefan Muetzel, Ron S. Ronimus, Kristy Lunn, Maik Kindermann, Stephane Duval, Michael Tavendale,