کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8498130 | 1553593 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The characterization and initial immune functional analysis of SCARA5 in turbot (Scophthalmus maximus L.)
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
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چکیده انگلیسی
Scavenger receptors (SRs) are a group of membrane-bound receptors that could bind to a variety of ligands including endogenous proteins and pathogens. SRs have been recognized to play vital roles in innate immune response against pathogen infection in both vertebrates and invertebrates. In this regard, one SmSCARA5 gene was captured in turbot (Scophthalmus maximus). The full-length SmSCARA5 transcript contains an open reading frame (ORF) of 1494 bp. SmSCARA55 showed both the highest identity and similarity to half-smooth tongue sole (Cynoglossus semilaevis), and a high degree of conservation of genomic structure to the teleost species. In addition, the phylogenetic tree analysis showed SmSCARA5 had the closest relationship to half-smooth tongue sole, the syntenic analysis revealed a relatively conserved synteny pattern of SmSCARA5 to other species. Moreover, SmSCARA5 was ubiquitously expressed in all the examined tissues, with the highest expression level in brain and the lowest expression level in blood. And it was significantly down-regulated in intestine following Gram-negative bacteria Vibrio anguillarum, and Gram-positive bacteria Streptococcus iniae challenge. Finally, the recombinant SmSCARA5 showed the highest affinity to lipopolysaccharide (LPS), followed by peptidoglycan (PGN) and lipoteichoic acid (LTA), as well as the strong inhibition effect on the growth of V. anguillarum. Taken together, our results suggested SmSCARA5 plays vital roles in innate immune response in teleost, further studies should be carried out to better understand its regulatory mechanism for innate inflammation response in teleost.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 81, October 2018, Pages 242-249
Journal: Fish & Shellfish Immunology - Volume 81, October 2018, Pages 242-249
نویسندگان
Chao Li, Mengyu Tian, Lu Zhang, Qiang Fu, Lin Song, Fei Chen, Ning Yang,