کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8498374 1553596 2018 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
RNA-seq based transcriptional analysis reveals dynamic genes expression profiles and immune-associated regulation under heat stress in Apostichopus japonicus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
RNA-seq based transcriptional analysis reveals dynamic genes expression profiles and immune-associated regulation under heat stress in Apostichopus japonicus
چکیده انگلیسی
In this study, we explored the gene expression profiles in Apostichopus japonicus under continuous heat stress (6 h, 48 h and 192 h) by applying RNA-seq technique. A total of 676, 1010 and 1083 differentially expressed genes were detected at three heat stress groups respectively, which suggested complex regulation of various biological processes. Then we focused on the changing of immune system under HS in sea cucumbers. Key immune-associated genes were involved in heat stress response, which were classified into six groups: heat shock proteins, transferrin superfamily members, effector genes, proteases, complement system, and pattern recognition receptors and signaling. Moreover, the mRNA expression of the immune-associated genes were validated by the real time PCR. Our results showed that an immunological strategy in this species was developed to confront abrupt elevated temperatures in the environment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 78, July 2018, Pages 169-176
نویسندگان
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