کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8501065 1553839 2018 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of an isothermal amplification-based assay for the rapid detection of Cronobacter spp.
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Development of an isothermal amplification-based assay for the rapid detection of Cronobacter spp.
چکیده انگلیسی
Cronobacter spp. is an opportunistic pathogen that is associated with rare but life-threatening neonatal infections resulting from the consumption of contaminated powdered infant formula milk (PIF). In the present study, we developed recombinase polymerase amplification (RPA) and real-time RPA for the detection of Cronobacter spp. in PIF for the first time by targeting the ompA gene. The specificity and sensitivity of the RPA and real-time RPA were validated and the practical applicability of these methods for the detection of Cronobacter spp. in artificially contaminated PIF samples was proved by comparing their reaction time, sensitivity, and efficacy with those of real-time PCR and the Chinese traditional method. The RPA and real-time RPA assays reduced the analysis time to less than 15 min and the results were as reliable as those of real-time PCR. Taken together, the RPA and real-time RPA assays served as fast, reliable, and sensitive techniques for the detection of Cronobacter spp.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 101, Issue 6, June 2018, Pages 4914-4922
نویسندگان
, , , , , , , ,