کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8504360 | 1554335 | 2017 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of silencing INHα gene by RNAi on the mRNA expressions of StAR, FST, INHβB, and FSHR genes in cultured sheep granulosa cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
The aim of the study was to investigate the effects of silencing the inhibin α-subunit (INHα) gene by RNAi on the mRNA levels of steroidogenic acute regulatory protein (StAR), follistatin (FST), inhibin β-subunit (INHβB), and FSH receptor (FSHR) in cultured sheep granulosa cells (GCs). The antral follicles, 3-7 mm in diameter, were dissected from the ovaries of 15 Thin-tailed Han sheep (1-1.5 years old), and then GCs were isolated from antral follicles. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to analyze the mRNA levels of StAR, FST, INHβB, and FSHR in INHα-specific small RNA interference (siRNA) â treated GCs. Knockdown of INHα mRNA by siRNA decreased the mRNA expressions of StAR and FST by 76% and 23%, respectively (P < 0.05), and increased the mRNA expressions of FSHR and INHβB by 81% and 50% in cultured sheep GCs, respectively (P < 0.05). These results indicated that silencing INHα gene by RNAi influenced the mRNA expressions of StAR, FST, INHβB and FSHR in cultured sheep GCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Small Ruminant Research - Volume 157, December 2017, Pages 23-26
Journal: Small Ruminant Research - Volume 157, December 2017, Pages 23-26
نویسندگان
T. Li, A.T. Ma, Y.J. Zhang, Y.Q. Liu, S.Y. Wang, X.F. Shi, K. Gao, C.H. Duan,