کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8528 596 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nanoporous gold film encapsulating cytochrome c for the fabrication of a H2O2 biosensor
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Nanoporous gold film encapsulating cytochrome c for the fabrication of a H2O2 biosensor
چکیده انگلیسی

A layer-by-layer route to prepare nanoporous Au film materials on transparent ITO substrates is reported by alternatively assembling Au and Ag nanoparticles through 1,5-pentanedithiol as a cross-linker, followed by that Ag nanoparticles are dissolved at room temperature in HAuCl4 solution. Electron transfer of cytochrome c (cyt. c) – an excellent model for investigation of biomolecules, is greatly facilitated at the nanoporous Au film with electron transfer rate constant (ks) of 3.9 s−1. Meanwhile, cyt. c adsorped onto the nanoporous Au film still maintain its enzymatic activity toward H2O2. On the basis of these experimental results, the cyt. c-nanoporous Au film is exploited to an amperometric biosensor for H2O2 with high selectivity, broad linear range, low detection limit, and long stability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 30, Issue 18, June 2009, Pages 3183–3188
نویسندگان
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