کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8528880 | 1558845 | 2018 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibition of microRNA-505 suppressed MPP+â¯-induced cytotoxicity of SHSY5Y cells in an in vitro Parkinson's disease model
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
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چکیده انگلیسی
Parkinson's disease (PD) is a progressive and incurable neurodegenerative disorder. In this study, we utilized SHSY5Y cells an in vitro PD model to study the biological function of microRNA-505 (miR-505) in 1-methyl-4-phenylpyridinium (MPP+)-induced cytotoxicity. SHSY5Y cells were treated with MPP+â¯to induce PD-associated cell death and cytotoxicity. MiR-505 was found to be upregulated by MPP+â¯. Lentiviral transduction mediated miR-505 downregulation protected SHSY5Y cells from MPP+â¯-induced cell death, whereas miR-505 upregulation had no effect on MPP+â¯-induced SHSY5Y cell death. MiR-505 downregulation also ameliorated MPP+â¯-induced cytotoxicity, by preserving MMP and reducing LDH level, caspase-3 and reactive oxygen species activities. Possible downstream target of miR-505, neural proliferation, differentiation and control 1 (NPDC1) was confirmed to be the downstream target of miR-505 in SHSY5Y cells. In addition, NPDC1 knockdown was shown to reverse the protection of miR-505-downregulation on MPP+â¯-induced cytotoxicity. Thus, our study demonstrated that miR-505, by inversely regulating NPDC1 may have a functional role in regulating human Parkinson's disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 835, 15 September 2018, Pages 11-18
Journal: European Journal of Pharmacology - Volume 835, 15 September 2018, Pages 11-18
نویسندگان
Jianzhong Zhu, Shan Wang, Yingyin Liang, Xue Xu,