کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8545916 | 1561685 | 2018 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of mancozeb, metalaxyl and tebuconazole on steroid production by bovine luteal cells in vitro
ترجمه فارسی عنوان
اثرات منکوز بی، متالاکسیل و تیبوکونازول بر تولید استروئید توسط سلول های لوتئال گاو در شرایط آزمایشگاهی
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
Mancozeb, metalaxyl and tebucanazole are widely used pesticides in agriculture and industry to treat plant pathogenic fungi. Livestock may be exposed to such substances by consuming contaminated plants. The present study was designed to evaluate the effects of these three fungicides on bovine luteal cell steroidogenesis. Luteal slices from mid-cycle corpus luteum were dissociated into single cell suspension in aerated (O2) culture media (DMEM/F12) by enzymatic digestion. The cells were incubated in newborn calf serum (10%) for 18â¯h and then with serum-free media containing mancozeb (0.01â¯Î¼M, 0.1â¯Î¼M, 1â¯Î¼M), tebuconazole (1â¯Î¼M, 10â¯Î¼M, 100â¯Î¼M) or metalaxyl (100â¯Î¼M, 500â¯Î¼M, 2500â¯Î¼M) for additional 96â¯h. The medium was replaced on day 1 and 3; and the retrieved medium was stored at â20â¯Â°C until progesterone assay. Treatment of cells with three different fungicides induced dose dependent variable decrease in steroid synthesis during incubation periods. Incubation of cells with 1â¯Î¼M mancozeb exhibited a 33% decline in steroid synthesis on day 3 and 48% decline on day 5 compared with controls. Treatment of cells with 100â¯Î¼M tebuconazole and 500â¯Î¼M metalaxyl resulted in a 65% and 31% decrease, respectively, in progesterone accumulation on day 5 of incubation. Fungicide induced suppressive effects on luteal steroidogenesis were as metalaxylâ¯<â¯tebuconazoleâ¯<â¯mancozeb. Results of the present study suggest that designated concentrations of all three fungicides studied might have varying degrees of adverse effects on luteal steroidogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 59, April 2018, Pages 114-118
Journal: Environmental Toxicology and Pharmacology - Volume 59, April 2018, Pages 114-118
نویسندگان
Nurgul Atmaca, Sevket Arikan, Dinc Essiz, Hakan Kalender, Ozkan Simsek, Fatih Sultan Bilmen, Ruhi Kabakci,