کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8546172 1561697 2016 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Role of miR-199b-5p in regulating angiogenesis in mouse myocardial microvascular endothelial cells through HSF1/VEGF pathway
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Role of miR-199b-5p in regulating angiogenesis in mouse myocardial microvascular endothelial cells through HSF1/VEGF pathway
چکیده انگلیسی
Our study explored effects of miR-199b-5p on angiogenesis in mouse myocardial microvascular endothelial cells (MMVECs) and the involved working mechanisms. We applied explant culture to incubate C57/BL6 mouse MMVECs. Lipofection was used to transfect miR-199b-5p mimic, miR-199b-5p inhibitor and miR-199b-5p scramble respectively. MMVECs were divided into miR-199b-5p up-regulation, miR-199b-5p down-regulation and control groups based on above sequence. Expressions of miR-199b-5p, heat shock factor protein 1 (HSF1) mRNA were assessed by real-time quantitative polymerase chain reaction (RT-QPCR). Expressions of HSF1 and vascular endothelial growth factor (VEGF) were assessed by Western Blotting. Cell proliferation was assessed by CCK8. Tubule formation assay was conducted to assess formation of blood vessels. Results showed that miR-199b-5p up/down-regulation groups exhibited no obvious differences in the expressions of HSF1 mRNA compared to control group. However, miR-199b-5p up-regulation group recorded lower expressions of HSF1 and VEGF in the level of protein, and reduced cell proliferation and tubule formation. Whereas, miR-199b-5p down-regulation group presented the contrary results. The experiment indicated that miR-199b-5p can regulate proliferation and angiogenesis in mouse MMVECs through the pathway of HSF1/VEGF.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 47, October 2016, Pages 142-148
نویسندگان
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