کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8624127 | 1567911 | 2018 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Shallow whole genome sequencing for robust copy number profiling of formalin-fixed paraffin-embedded breast cancers
ترجمه فارسی عنوان
توالی کامل ژنوم کل کامل برای اندازه کپی عظیم پروفایل سرطان پستان جاسازی شده با پارافین ثابت شده
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی بالینی
چکیده انگلیسی
Pathology archives with linked clinical data are an invaluable resource for translational research, with the limitation that most cancer samples are formalin-fixed paraffin-embedded (FFPE) tissues. Therefore, FFPE tissues are an important resource for genomic profiling studies but are under-utilised due to the low amount and quality of extracted nucleic acids. We profiled the copy number landscape of 356 breast cancer patients using DNA extracted FFPE tissues by shallow whole genome sequencing. We generated a total of 491 sequencing libraries from 2 kits and obtained data from 98.4% of libraries with 86.4% being of good quality. We generated libraries from as low as 3.8â¯ng of input DNA and found that the success was independent of input DNA amount and quality, processing site and age of the fixed tissues. Since copy number alterations (CNA) play a major role in breast cancer, it is imperative that we are able to use FFPE archives and we have shown in this study that sWGS is a robust method to do such profiling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental and Molecular Pathology - Volume 104, Issue 3, June 2018, Pages 161-169
Journal: Experimental and Molecular Pathology - Volume 104, Issue 3, June 2018, Pages 161-169
نویسندگان
Suet-Feung Chin, Angela Santonja, Marta Grzelak, Soomin Ahn, Stephen-John Sammut, Harry Clifford, Oscar M. Rueda, Michelle Pugh, Mae A. Goldgraben, Helen A. Bardwell, Eun Yoon Cho, Elena Provenzano, Federico Rojo, Emilio Alba, Carlos Caldas,