کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8624229 1567914 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Matrix metalloproteinase and heparin-stimulated serine proteinase activities in post-prostate massage urine of men with prostate cancer
ترجمه فارسی عنوان
فعالیت های متالوپروتئیناز ماتریکس و فعالیت پروتئیناز سریالی در ادرار ماساژ پس از پروستات مردان مبتلا به سرطان پروستات
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
چکیده انگلیسی
Proteinases secreted by the prostate gland have a reproductive function in cleaving proteins in the ejaculate and in the female reproductive tract, but some may have a fundamental role in disease and pathological processes including cancer. The purpose of this study was to determine if there were differences in proteinase activities in urine samples collected following prostate massage of men positive (CaP) or negative (no evidence of malignancy, NEM) for biopsy determined prostate cancer. Matrix metalloproteinase (MMP) and serine proteinase activities were detected using protein substrate zymography. There were no differences in activities of MMP-2, proMMP-9, and MMP-9/NGAL (neutrophil gelatinase associated lipocalin) complex (gelatin substrate) in men with detected prostate cancer, although the latter two were somewhat diminished. A caseinolytic activity of about 75 kDa inhibited by calcium did not differ between the NEM and CaP groups. Heparin stimulated calcium sensitive gelatinolytic activities of approximately 22, 42, and 60 kDa, but did not affect activities of MMP-2, MMP-9, or the 75 kDa caseinolytic activity. The 22, 42, and 60 kDa activities appear to be serine proteinases since they were inhibited by benzamidine. There was a significant decrease in the 22 kDa heparin-stimulated serine proteinase activity in urines of men with cancer. Proteinase expression and activities, perhaps in combination with other potential markers, may prove useful in urine for detection and evaluation of prostate cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental and Molecular Pathology - Volume 103, Issue 3, December 2017, Pages 300-305
نویسندگان
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