کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8627500 | 1568663 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of pro-apoptotic markers is increased along the osteochondral junction in naturally occurring osteochondrosis
ترجمه فارسی عنوان
بیان علامتهای پروپوپتوتیک در طول اتصال استئوآندرول در استئوکندروزی طبیعی افزایش می یابد
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کلمات کلیدی
TNFPOABAG-1LCMBcl-2Quarter Horse - اسب چهارمOsteochondrosis - استئوکاندروزoptimal cutting temperature compound - ترکیب دما برش مطلوبCartilage development - توسعه غضروفOsteochondritis dissecans - تومورهای استئوآرتریتThoroughbred - خالصApoptosis - خزان یاختهایCartilage biology - زیست شناسی غضروفtumor necrosis factor - فاکتور نکروز تومورB-cell lymphoma 2 - لنفوم سلول B 2laser capture microdissection - لیزر ضبط میکرو دیسکسیونEndochondral ossification - پوکی استخوان اندوخوردار
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
چکیده انگلیسی
Osteochondrosis (OC) is a naturally occurring disease of the articular-epiphyseal cartilage and subchondral bone layers, leading to pain and decreased mobility. The objective of this study was to characterize gene and protein expression of apoptotic markers in chondrocytes surrounding cartilage canals and along the osteochondral junction of osteochondrosis (OC)-affected and normal cartilage, using naturally occurring disease in horses. Paraffin-embedded osteochondral samples (6 OC, 8 normal controls) and cDNA from chondrocytes captured with laser capture microdissection (4 OC, 6 normal controls) were obtained from the lateral trochlear ridge of femoropatellar joints in 14 immature horses (1-6â¯months of age). Equine-specific caspase-3, caspase-8, caspase-10, Fas, Bcl-2, BAG-1, TNFα, cytochrome C, thymosin-β10, and 18S mRNA expression levels were evaluated by two-step real-time quantitative PCR. Percentage of cell death was determined using the TUNEL method. Protein expression of caspase-10, Fas, cytochrome C, and thymosin-β10 was determined following immunohistochemistry. Statistical analysis was performed using the Wilcoxon rank sum test or two-sample t-test (pâ¯<â¯0.05). In OC samples, there was significantly increased gene expression of caspase-10, Fas, cytochrome C, and thymosin-β10 in chondrocytes along the osteochondral junction and increased Fas gene expression in chondrocytes adjacent to cartilage canals, compared to controls. In OC samples, higher matrix Fas and cytochrome C protein expression, lower mitochondrial cytochrome C protein expression, and a trend for higher cytoplasmic caspase-10 protein expression were found. Collectively, these results suggest that both extrinsic and intrinsic apoptotic pathways are activated in OC cartilage. Increased apoptosis of osteochondral junction chondrocytes may play a role in OC, based on increased gene expression of several pro-apoptotic markers in this location.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone Reports - Volume 9, December 2018, Pages 19-26
Journal: Bone Reports - Volume 9, December 2018, Pages 19-26
نویسندگان
Stacy A. Semevolos, Katja F. Duesterdieck-Zellmer, Maureen Larson, Marc A. Kinsley,