کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8644482 | 1569761 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel prognostic biomarker for pancreatic ductal adenocarcinoma: hsa_circ_0001649
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کلمات کلیدی
CCK-8SHPRHATCCHPDERT-qPCRhsa_circ_0001649PBSCircular RNA - RNA دایره ایcircular RNAs - RNA های دایره ایPancreatic ductal adenocarcinoma - آدنوکارسینوم پانکراس داکتالPDAC یا pancreatic ductal adenocarcinoma - آدنوکارسینومای داکتال پانکراسstandard deviation - انحراف معیارBiomarker - بیومارکرCompeting endogenous RNA - رقابت RNA درونیRIN - رینcircRNAs - سیرک هاceRNA - سینهcell counting kit-8 - شمارش سلول کیت 8RNA Integrity Number - شماره یکپارچه RNAPhosphate buffered saline - فسفات بافر شورAmerican Type Culture Collection - مجموعه فرهنگی نوع آمریکاییreal-time quantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استPropidium iodide - پروتئین یدید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease with increasing incidence worldwide. Accumulating evidence indicated that circular RNAs (circRNAs) behave as a novel class of transcription products during multiple cancer processes. Specifically, hsa_circ_0001649 has been reported to be down-regulated in several cancers. However, its clinical significance and functional roles in PDAC is still unknown. RT-qPCR was carried out to measure the expression of hsa_circ_0001649 in PDAC tissue samples and cell lines. Additionally, the correlation between hsa_circ_0001649 expression and clinicopathological features was analyzed. The prognostic role of hsa_circ_0001649 was explored by Cox regression analysis. The potential effects of hsa_circ_0001649 in PDAC cells were evaluated in vitro including cell proliferation, colony-forming ability and apoptosis. As a result, hsa_circ_0001649 was abnormally decreased in PDAC tissues and cells, and this down-regulation was correlated with tumor stage and differentiation grade in PDAC patients. Hsa_circ_0001649 could serve as an independent prognostic factor for PDAC patients after surgery. What's more, increased hsa_circ_0001649 caused tumor suppressive effects via reducing cell proliferation, colony-forming ability and promoting cell apoptosis in PANC1 and BxPC3 cells. Collectively, the results illustrated that hsa_circ_0001649 may play a tumor suppressor role in PDAC and offer a potential therapeutic target for treating this fatal disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 675, 30 October 2018, Pages 88-93
Journal: Gene - Volume 675, 30 October 2018, Pages 88-93
نویسندگان
Yuehong Jiang, Tong Wang, Li Yan, Lijuan Qu,