کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8644989 | 1569773 | 2018 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
miR-758 mediates oxLDL-dependent vascular endothelial cell damage by suppressing the succinate receptor SUCNR1
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کلمات کلیدی
HUVECSGPR91SUCNR1Dlk1oxLDLp-STAT3DAPI3′ UTR - 3 UTR3′ untranslated region - 3 منطقه غیر ترجمه4,6-diamidino-2-phenylindole - 4،6-دیامیدین-2-فنیلینولMTT - MTTROS/RNS - ROS / RNSDio3 - دیو 3Human vascular endothelial cells - سلول های اندوتلیال عروقی انسانVascular endothelial cells - سلولهای اندوتلیال عروقیphosphorylated STAT3 - فسفریک شده STAT3Oxidized low-density lipoprotein - لیپوپروتئین با چگالی کم اکسید شدهmethyl thiazolyl tetrazolium - متیل تیزولیل تترازولیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Atherosclerosis is a vascular disease associated with ageing, and its occurrence and development are closely related to the vascular inflammatory response. Oxidized low-density lipoprotein (oxLDL) has distinct effects in atherosclerosis. We aimed to determine the mechanisms underlying these effects. microRNAs including miR-758 were differentially expressed in oxLDL-treated HUVECs or HAECs. Luciferase reporter assay results indicated that SUCNR1 is an important target of miR-758. Expression of SUCNR1 and its downstream components was decreased significantly in ApoEâ/â mice. Overexpression of miR-758 could suppress HUVEC proliferation by cell cycle arrest at the G0/G1 phase. miR-758 was overexpressed on HUVECs with markedly reduced capillary tubule formation capacity. Overexpression of miR-758 on HUVECs or HAECs could significantly reduce SUCNR1 (GPR91), SATA3, phosphorylated STAT3 (p-STAT3), and EVGF levels. Thus, oxLDL likely damages vascular endothelial cells by modulating the DLK1-DIO3 genomic imprinted microRNA cluster component miR-758, thereby suppressing expression of SUCNR1/GPR91 and its downstream components.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 663, 15 July 2018, Pages 1-8
Journal: Gene - Volume 663, 15 July 2018, Pages 1-8
نویسندگان
Hu Zhang, Jiajia Zheng, Jiajia Lin, Jiulin Chen, Zhihua Yu, Chuan Chen, Te Liu,