Regulation of lipid metabolism in adipose depots of fat-tailed and thin-tailed lambs during negative and positive energy balances

This study aimed to evaluate the effects of negative and positive energy balances on gene expression of regulators and enzymes controlling lipogenesis and lipolysis in muscle and adipose depots of fat-tailed and thin-tailed lambs. Lambs were slaughtered during neutral, negative and positive energy balances for sample collection. Real time q-PCR was conducted to measure the gene expression. Expression of PPARγ was increased in response to positive energy balance regardless of genotype and type of tissue (P < 0.04). Expression of SREBF1 was reduced in response to negative and positive energy balances in fat-tailed lambs, whereas in thin-tailed lambs, downregulated SREBF1 was restored during positive energy balance (P < 0.01). Enhancement in FABP4 expression in response to negative and positive energy balances was respectively higher in thin-tailed and fat-tailed lambs affected by interaction of genotype and energy balance (P < 0.11). In thin-tailed lambs, the enhanced FABP4 expression in response to negative energy balance was considerably higher in mesenteric adipose depot, whereas in fat-tailed lambs, positive energy balance induced enhancement in FABP4 expression was considerably higher in fat-tail adipose depot. The results demonstrate that transcription regulation of lipogenesis and lipolysis during negative and positive energy balances occurs differently in fat-tailed and thin-tailed lambs. Thin-tailed and fat-tailed lambs are respectively more responsive to negative and positive energy balances and mesenteric and fat-tail adipose depots respectively in thin-tailed and fat-tailed lambs are the main adipose depots responsible for higher responsiveness of thin-tailed and fat-tailed lambs to negative and positive energy balances.