کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8648435 | 1570692 | 2018 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of the mature form of a β-defensin-like peptide, Hoa-D1, in the lobster Homarus americanus
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
Homarus americanusNanoLCQTOF-MS/MSDefensinTICnanoESIMWCODTTCIDTSAAMPIAAEST - استcollision induced dissociation - تقارن ناشی از برخوردExpressed Sequence Tag - تکرار اظهار نظرdithiothreitol - دیتیوتریتولTranscriptomics - متن ترانهEIC - مهندسانTranscriptome shotgun assembly - مونتاژ تفنگ ساچمه ایHemolymph - همولومیفMolecular Weight Cut Off - وزن مولکولی کاهش یافته استAntimicrobial peptide - پپتیدهای ضدمیکروبیNano-liquid chromatography - کروماتوگرافی نانو مایعextracted ion chromatogram - کروماتوگرافی یون استخراج شدهNanoelectrospray ionization - یونیزاسیون نانو الکترو پراشiodoacetamide - یووداکتامید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We report on the characterization of the native form of an American lobster, Homarus americanus, β-defensin-like putative antimicrobial peptide, H. americanus defensin 1 (Hoa-D1), sequenced employing top-down and bottom-up peptidomic strategies using a sensitive, chip-based nanoLC-QTOF-MS/MS instrument. The sequence of Hoa-D1 was determined by mass spectrometry; it was found to contain three disulfide bonds and an amidated C-terminus. The sequence was further validated by searching publicly-accessible H. americanus expressed sequence tag (EST) and transcriptome shotgun assembly (TSA) datasets. Hoa-D1, SYVRScSSNGGDcVYRcYGNIINGAcSGSRVccRSGGGYamide (with c representing a cysteine participating in a disulfide bond), was shown to be related to β-defensin-like peptides previously reported from Panulirus japonicas and Panulirus argus. We found Hoa-D1 in H. americanus hemolymph, hemocytes, the supraoesophageal ganglion (brain), eyestalk ganglia, and pericardial organ extracts, as well as in the plasma of some hemolymph samples. Using discontinuous density gradient separations, we fractionatated hemocytes and localized Hoa-D1 to hemocyte sub-populations. While Hoa-D1 was detected in semigranulocytes and granulocytes using conventional proteomic strategies for analysis, the direct analysis of cell lysates exposed evidence of Hoa-D1 processing, including truncation of the C-terminal tyrosine residue, in the granulocytes, but not semigranulocytes. These measurements demonstrate the insights regarding post-translational modifications and peptide processing that can be revealed through the MS analysis of intact peptides. The identification of Hoa-D1 as a widely-distributed peptide in the lobster suggests the possibility that it may be pleiotropic, with functions in addition to its proposed role as an antimicrobial molecule in the innate immune system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 101, September 2018, Pages 329-343
Journal: Molecular Immunology - Volume 101, September 2018, Pages 329-343
نویسندگان
Giap H. Vu, Daniel Do, Cindy D. Rivera, Patsy S. Dickinson, Andrew E. Christie, Elizabeth A. Stemmler,