کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
870555 1471020 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of mycophenolic acid by Penicillium brevicompactum—A comparison of two methods of optimization
موضوعات مرتبط
مهندسی و علوم پایه مهندسی انرژی انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
پیش نمایش صفحه اول مقاله
Production of mycophenolic acid by Penicillium brevicompactum—A comparison of two methods of optimization
چکیده انگلیسی


• Medium optimization for MPA production using P. brevicompactum by one-factor-at-a-time and CCD methods.
• CCD afforded a 40% higher MPA titer than one-factor-at-a-time method.
• The titer was nearly 6-fold higher compared to un-optimized medium.

Production of mycophenolic acid (MPA) by submerged fermentation using the microfungus Penicillium brevicompactum MTCC 8010 is reported here. Screening experiments were used to identify: the suitable media composition; the optimal initial pH; and the optimal incubation temperature to maximize the production of MPA in batch cultures. The initial concentrations of the selected sources of carbon (glucose), nitrogen (peptone) and the precursors (methionine, glycine) were then optimized by: (1) one-at-a-time variation of factors; and (2) a central composite design (CCD) of experiments, in a 12-day batch culture at an initial pH of 5.0, an incubation temperature of 25 °C, and an agitation speed of 200 rpm. The medium optimized using the one-at-a-time variation yielded a peak MPA titer of 1232 ± 90 mg/L. The medium optimized by the CCD method yielded a 40% higher MPA titer of 1737 ± 55 mg/L. The latter value was nearly 9-fold greater than the titer achieved prior to optimization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biotechnology Reports - Volume 11, September 2016, Pages 77–85
نویسندگان
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