کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
870556 | 1471020 | 2016 | 4 صفحه PDF | دانلود رایگان |
• The recombinant human PP2A catalytic subunit (rhPP2Ac) was produced in the baculovirus expression system with High Five insect cells.
• The expression at 19 °C can produce the rhPP2Ac with a higher activity and in a larger quantity than in the incubation conducted at 27 °C.
• To examine the effects of the low temperature expression on other phosphatases, we expressed human PP2B and PP2C in High Five insect cells.
• Optimizing the expression temperature in a baculovirus system is effective for producing a recombinant protein.
Protein phosphatase 2A (PP2A) is an enzyme useful for detecting several natural toxins represented by okadaic acid and microcystins. We found that the production of the recombinant human PP2A catalytic subunit (rhPP2Ac) in High Five insect cells could markedly increase when the cells were cultured at 19 °C instead of 27 °C used under conventional conditions. The yield and purity of the enzyme increased four- and three-folds, respectively. The benefit of the altered culturing temperature was observed with the recombinant human protein phosphatase 2B but not 2Cα. The different responses among the enzymes suggest the involvement of an enzyme-specific mechanism that leads to the catalytic subunit overexpression. This is the first report to produce rhPP2Ac at a temperature lower than that used under conventional culture conditions (27 °C) used in the baculovirus expression system with High Five insect cells.
Journal: Biotechnology Reports - Volume 11, September 2016, Pages 86–89