کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
870594 1471023 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Heterologous overexpression of sfCherry fluorescent protein in Nannochloropsis salina
موضوعات مرتبط
مهندسی و علوم پایه مهندسی انرژی انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
پیش نمایش صفحه اول مقاله
Heterologous overexpression of sfCherry fluorescent protein in Nannochloropsis salina
چکیده انگلیسی


• Heterologous sfCherry protein was expressed in N. salina for the first time.
• N. salina was transformed by particle bombardment.
• Integration site of the transgene on the genome was determined by RESDA PCR.
• Expression of sfCherry was confirmed by a western blotting and confocal microscopy.

Oleaginous microalgae of the Nannochloropsis genus are considered excellent candidates for biofuels and value-added products owing to their high biomass productivity and lipid content. Here, we report the first overexpression and detection of a heterologous sfCherry fluorescent protein in Nannochloropsis salina in order to develop a transformation toolbox for future genetic improvements. Particle bombardment was employed for transformation, and expression of Shble under the control of TUB and UEP promoters, cloned from N. salina, was used to confer resistance to Zeocin antibiotics, resulting in 5.9 and 4.7 transformants per 108 cells, respectively. Stable integration of the markers into the genome was confirmed using a restriction enzyme site-directed amplification (RESDA) PCR. The expression of sfCherry fluorescent protein was confirmed by Western blot analysis and confocal microscopy. These results suggest new possibilities of efficient genetic engineering of Nannochloropsis for the production of biofuels and other biochemicals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biotechnology Reports - Volume 8, December 2015, Pages 10–15
نویسندگان
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