کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
872728 910279 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dynamic mechanical properties of the tissue-engineered matrix associated with individual chondrocytes
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی پزشکی
پیش نمایش صفحه اول مقاله
Dynamic mechanical properties of the tissue-engineered matrix associated with individual chondrocytes
چکیده انگلیسی

The success of cell-based tissue engineering approaches in restoring biological function will be facilitated by a comprehensive fundamental knowledge of the temporal evolution of the structure and properties of the newly synthesized matrix. Here, we quantify the dynamic oscillatory mechanical behavior of the engineered matrix associated with individual chondrocytes cultured in vitro for up to 28 days in alginate scaffolds. The magnitude of the complex modulus (|E*|) and phase shift (δ) were measured in culture medium using Atomic Force Microscopy (AFM)-based nanoindentation in response to an imposed oscillatory deformation (amplitude ∼5 nm) as a function of frequency (f=1–316 Hz), probe tip geometry (2.5 μm radius sphere and 50 nm radius square pyramid), and in the absence and presence of growth factors (GF, insulin growth factor-1, IGF-1, and osteogenic protein-1, OP-1). |E*| for all conditions increased nonlinearly with frequency dependence approximately f1/2 and ranged between ∼1 and 25 kPa. This result, along with theoretical calculations of the characteristic poroelastic relaxation frequency, fp, (∼50–90 Hz) suggested that this time-dependent behavior was governed primarily by fluid flow-dependent poroelasticity, rather than flow-independent viscoelastic processes associated with the solid matrix. |E*(f)| increased, (f) decreased, and the hydraulic permeability, k, decreased with time in culture and with growth factor treatment. This trend of a more elastic-like response was thought to be associated with increased macromolecular biosynthesis, density, and a more mature matrix structure/organization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biomechanics - Volume 43, Issue 3, 10 February 2010, Pages 469–476
نویسندگان
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