کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8739120 1592027 2018 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of Mycobacterium tuberculosis-derived DNA in circulating cell-free DNA from a patient with disseminated infection using digital PCR
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Detection of Mycobacterium tuberculosis-derived DNA in circulating cell-free DNA from a patient with disseminated infection using digital PCR
چکیده انگلیسی
Mycobacterium tuberculosis (MTB) can disseminate to extrapulmonary organs, particularly in severely immunosuppressed patients. Confirmation of active MTB infection is often difficult in subjects with a contraindication for invasive procedures. A case of disseminated MTB infection after hematopoietic stem cell transplantation is reported herein. Circulating cell-free DNA from the patient showed positive amplification of an MTB complex-specific sequence using a digital PCR technique. The MTB infection was confirmed by positive acid-fast staining and an approved quantitative PCR assay using liver tissue obtained at autopsy. The detection of MTB in circulating cell-free DNA using this technique may represent a less invasive diagnostic tool for pulmonary and extrapulmonary MTB infections.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Infectious Diseases - Volume 66, January 2018, Pages 80-82
نویسندگان
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