A “Ct contrast”-based strain-specific real-time quantitative PCR system for Lactobacilllus paracasei subsp. paracasei NTU 101

The two-component, “Ct contrast” approach is useful for qPCR discrimination when no ideal PCR target is available or the variance of the target site is unpredictable. The Ct contrast approach might provide a simple and robust solution for other challenging qPCR targets.