A SYBR® Green-based real-time PCR method for improved detection of mcr-1-mediated colistin resistance in human stool samples

A rapid rt-PCR for detection of mcr-1 from stool specimens was developed. The detection rate was increased by testing selective broth enrichments. This approach also has the advantage of concomitant isolation of mcr-1-harboring strains for further antimicrobial susceptibility and genetic testing.