کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8759607 | 1596886 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
miR-17-92 ameliorates renal ischemia reperfusion injury
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی و دندانپزشکی (عمومی)
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چکیده انگلیسی
There is limited information on the role of miR-17-92 in renal tubular pathophysiology. Therefore, the present study was performed to determine whether miR-17-92 plays a role in ischemia-reperfusion injury (IRI)-induced acute kidney injury. We originally demonstrated that miR-17-92 is up-regulated following IRI in vivo. To explore the roles of miR-17-92 in the IRI process, we first generated a renal proximal tubule-specific miR-17-92 deletion (PT-miR-17-92â/â) knockout mouse model with Cre driven by the Kap promoter. We found that PT-deficient miR-17-92 mice had more severe renal dysfunction and renal structures than their littermates. Compared with sham-operated mice, both wide-type (WT) mice and PT-miR-17-92â/â mice showed increased serum levels of creatinine and urea. However, the levels of serum urea and creatinine in PT-miR-17-92â/â mice after the IRI operation were significantly higher than the levels in WT mice. In addition, PT-miR-17-92â/â mice showed higher levels of serum potassium and phosphonium after the IRI operation. Histological analysis revealed that PT-miR-17-92â/â mice had substantial histopathologic changes, such as tubular dilation and tubular necrosis. Overexpression of miR-17-92 could partially reverse the side-effects of IRI on the proximal tubules in vivo. Furthermore, we employed a quantitative proteomic strategy and identified 16 proteins as potential targets of miR-17-92. Taken together, our findings suggested that miR-17-92 may ameliorates IRI-induced acute kidney injury. Our results indicate that pharmacologic modulation of these miRNAs may have therapeutic potential for acute kidney injury.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Kaohsiung Journal of Medical Sciences - Volume 34, Issue 5, May 2018, Pages 263-273
Journal: The Kaohsiung Journal of Medical Sciences - Volume 34, Issue 5, May 2018, Pages 263-273
نویسندگان
Turun Song, Mianzhi Chen, Zhengsheng Rao, Yang Qiu, Jinpeng Liu, Yamei Jiang, Zhongli Huang, Xianding Wang, Tao Lin,