کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
877504 911030 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Disassembly and reassembly improves morphology and thermal stability of human papillomavirus type 16 virus-like particles
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی پزشکی
پیش نمایش صفحه اول مقاله
Disassembly and reassembly improves morphology and thermal stability of human papillomavirus type 16 virus-like particles
چکیده انگلیسی

Recombinant human papillomavirus (HPV) 16 L1 protein self-assembles into virus-like particles (VLPs) with diameters of 40 to 60 nm, which are key components in prophylactic HPV vaccines. Marked improvement in morphology and thermal stability on VLP disassembly and reassembly was demonstrated at production scale. Differential scanning calorimetry showed enhanced conformational stability as indicated by the unfolding temperatures and peak heights/areas. Cloud point studies indicated (1) a much lower propensity for post-reassembly VLPs to aggregate during a time course study and (2) much higher cloud point temperatures. In-solution atomic force microscopy showed more uniform size distribution and fully closed particles, with evidence of virion-like assembly revealed by the structural details from a single particle image. Similar approaches for the reassembly of other recombinant VLPs with intrinsic conformational switches would be expected to improve the particle properties and render nanoparticles more suitable for use as vaccines or therapeutics.From the Clinical EditorThe authors of this study demonstrated that recombinant human papillomavirus 16 L1 protein self-assembles into virus-like particles (VLPs) with marked improvement in morphology and thermal stability on VLP disassembly and reassembly at production scale. This is expected to render these nanoparticles more suitable for use as vaccines or therapeutics.

Graphical AbstractIn-solution AFM characterization of HPV VLP size distribution, morphology and surface features.Figure optionsDownload high-quality image (386 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nanomedicine: Nanotechnology, Biology and Medicine - Volume 8, Issue 7, October 2012, Pages 1182–1189
نویسندگان
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