Enhanced antimicrobial activity of silver nanoparticles synthesized by Cryphonectria sp. evaluated singly and in combination with antibiotics

The newly identified pathogenic species of the genus Cryphonectria was exploited for the synthesis of silver nanoparticles (AgNPs). The extracellular synthesis of nanoparticles was fast and eco-friendly. These nanoparticles were analyzed and determined by ultraviolet–visible spectrophotometry, which detected AgNPs in the solution, and by NanoSight LM20, which determined their average size and concentration. The synthesized AgNPs showed a little monodispersity in the range of 30–70 nm with a concentration of 6.82 × 108 particles per milliliter of solution. The AgNPs demonstrated antibacterial activity against Staphylococcus aureus, Escherichia coli, Salmonella typhi, and Candida albicans, with comparatively higher activity against both S. aureus and E. coli than against S. typhi and C. albicans, which showed the lowest activity. The present study demonstrates the possible use of biologically synthesized AgNPs in the field of medicine. Thus, Cryphonectria sp. could be used for simple, nonhazardous, and efficient synthesis of AgNPs.From the Clinical EditorIn this study Cryphonectria sp. was exploited for simple, nonhazardous, and efficient biosynthesis of silver nanopartricles, which had antibacterial activity against a variety of human pathogenic bacteria.

Graphical AbstractA biological approach for the synthesis of bioactive silver nanoparticles using filtrate of Cryphonectria sp. has been developed. The filtrate was responsible for the reduction of aqueous silver ions. These silver nanoparticles demonstrated remarkable activity against multi-drug-resistant microorganisms, viz., Staphylococcus aureus, Escherichia coli, Salmonella typhi and Candida albicans.Figure optionsDownload high-quality image (83 K)Download as PowerPoint slide