Preparation and characterization of recombinant human growth hormone–Zn2+-dextran nanoparticles using aqueous phase–aqueous phase emulsion

An effective and simple method was developed to prepare spherical and uniform-sized recombinant human growth hormone (rhGH)–Zn2+-dextran nanoparticles (NPs) for a variety of delivery applications of the delicate protein. The results showed that the prepared rhGH-Zn2+-dextran particles were spherical, glassy, and uniform in size. rhGH recovered from the NPs maintained its structural integrity and its bioactivity well, as suggested by the Nb2-11 cell proliferation assay. Using polyethylene glycol and polysaccharide to mediate Zn2+-induced precipitation has proved to be a promising method to prepare spherical protein-loaded NPs with desired sizes and preserved protein stability.From the Clinical EditorThis basic science paper discusses a simple yet effective method to prepare spherical and uniform-sized recombinant human growth hormone (rhGH)-Zn2+-dextran nanoparticles for a variety of delivery applications.

Graphical AbstractThe objective of the study was to develop an rhGH-Zn2+-dextran nanoparticulate system with high encapsulating efficiency and preserved protein stability using two aqueous phase polymer solution emulsion of PEG solution and dextran solution, through being added rhGH and Zn2+ solution. The results showed that uniform size of rhGH-Zn2+-dextran spherical nanoparticles with high encapsulation efficiency and Nb2-11 cell proliferation activity were achieved.Figure optionsDownload high-quality image (151 K)Download as PowerPoint slide