کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8792105 | 1602570 | 2016 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optical monitoring of retinal respiration in real time: 670Â nm light increases the redox state of mitochondria
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی و میکروب شناسی (عمومی)
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چکیده انگلیسی
Mitochondria play a key role in ageing and disease. Their membrane potentials and ATP production decline with age and this is associated with progressive inflammation, cell loss and death. Here we use broadband Near-Infrared Spectroscopy (NIRS) to non-invasively measure in-vivo changes in aged retinal mitochondrial respiration following exposure to 670Â nm, which improves mitochondrial performance and reduces inflammation. Low power NIR light was shone into the eye via a fibre optic and the reflection monitored to measure signature changes in the oxidation of cytochrome c oxidase (COX) in complex IV of the electron transport chain. Changes in retinal haemodynamics and oxygenation were also recorded simultaneously with COX by measuring changes in oxygenated and deoxygenated haemoglobin (Î[HbO2] and Î[HHb]). Retinae of aged rats exposed to 670Â nm for 5Â mins showed consistent progressive increases in oxidation of COX 5Â mins post exposure. This remained significantly greater than baseline for up to 2Â h. This was not seen when retinae were exposed to 420Â nm light of the same power or when no light was applied. 670Â nm exposure significantly increased total haemoglobin concentration (Î[HbT]Â =Â Î[HbO2]Â +Î[HHb]) but not haemoglobin difference (Î[HbDiff]Â =Â Î[HbO2] -Î[HHb]). There were no changes in blood metrics in association with 420Â nm light or when no light exposure was given. Hence, brief 670Â nm exposure that is associated with reduced inflammation has a significant positive impact on the redox state of COX in aged retinae. The relative redox state of retinal COX may provide a valuable biomarker in ageing and macular degeneration where declining mitochondrial function is implicated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 152, November 2016, Pages 88-93
Journal: Experimental Eye Research - Volume 152, November 2016, Pages 88-93
نویسندگان
Pardis Kaynezhad, Ilias Tachtsidis, Glen Jeffery,