Establishment of a Quantitative Polymerase Chain Reaction Assay for Monitoring Chimeric Antigen Receptor T Cells in Peripheral Blood

The assay had a minimum detection limit of 10 copies/μL and a strong linear standard curve (y = −3.3682x + 38.594; R2 = 0.999) within the range of the input CAR gene (10-107 copies/μL). The reproducibility test showed a coefficient of variation ranging from 0.63%-1.65%. Real-time qPCR is a highly sensitive, specific, reproducible, and universal method that can be used to detect anti-CD19 CAR-T cells in peripheral blood.