کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8839057 | 1613221 | 2018 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Connexin43, but not connexin30, contributes to adult neurogenesis in the dentate gyrus
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کلمات کلیدی
PBS5-bromo-2-deoxyuridineGCLSGZaCSFSVZβ-galPFAAMPAα-amino-3-hydroxy-5-methylisoxazole-4-propionic acidZO-1eGFPCX43GFAPstratum oriensCA1Sulforhodamine 101stratum radiatum - stratat radiatumAstrocyte - آستروسیتcornu Ammonis 1 - آمون شاخ 1gamma-aminobutyric acid - اسید گاما آمینو بوتیریکstratum pyramidale - اهرام قهرمانβ-galactosidase - بتا گالاکتوزیدازBrdU - بروموداکسی اوریدینRoom temperature - دمای اتاقdentate gyrus - شکنج دندانه دارgap junction - فاصله ی شکافgranule cell layer - لایه سلول گرانولartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریsubgranular zone - منطقه غده گرانولیsubventricular zone - منطقه فرعیZonula occludens-1 - نوار ابزار بسته 1wild-type - نوع وحشیHippocampus - هیپوکامپ paraformaldehyde - پارافرمالدهیدGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استconnexin - کنسکسینGABA - گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
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چکیده انگلیسی
The subgranular zone of the dentate gyrus represents a niche in which radial glia (RG)-like cells generate new neurons throughout postnatal life in the mammalian brain. Previous data showed that RG-like cells are coupled through gap junction channels, primarily formed by connexin43 (Cx43) and Cx30, and that the expression of these proteins is required for adult neurogenesis in the hippocampus. However, their individual function and underlying mechanisms remain unclear. Here we demonstrate that Cx43, but not Cx30, is crucial for adult neurogenesis. To assess whether Cx43-dependent intercellular coupling between RG-like cells or rather channel-independent interactions of the protein regulate neurogenesis, mice bearing a Cx43 point mutation (Cx43G138R) in RG-like cells and protoplasmic astrocytes cells were employed, which was expected to cause channel closure without affecting the trafficking of the protein to the membrane. We confirmed the disruption of coupling between RG-like cells and astrocytes in the hippocampus of Cx43G138R mice. Proliferative activity and neurogenesis in the DG were significantly decreased in the mutant mouse line, indicating that functional Cx43 channels are essential for proper adult neurogenesis. The fate of proliferating cells in the DG was not affected by Cx43 mutation as revealed by 5-bromo-2-deoxyuridine (BrdU) incorporation assays. Together, these findings suggest that adult neurogenesis in the hippocampus does not require Cx30 but channel-dependent functions of Cx43.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research Bulletin - Volume 136, January 2018, Pages 91-100
Journal: Brain Research Bulletin - Volume 136, January 2018, Pages 91-100
نویسندگان
Jiong Zhang, Stephanie Griemsmann, Zhou Wu, Radoslaw Dobrowolski, Klaus Willecke, Martin Theis, Christian Steinhäuser, Peter Bedner,