کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8840595 | 1614691 | 2018 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Confirmation of Luteinizing Hormone (LH) in Living Human Vitreous and the Effect of LH Receptor Reduction on Murine Electroretinogram
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کلمات کلیدی
PBSLHRPDRTBSTLLOQECLMSDERGBSA - BSAbovine serum albumin - آلبومین سرم گاوelectroretinography - الکتروترینگرافیElectrochemiluminescence - الکتروکمی لومینسانسHCG - اچ سی جیProliferative diabetic retinopathy - رتینوپاتی دیابتی پرولیفراتیوVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Photoreceptors - فتوتراپیlower limit of quantitation - محدودیت پایین کمیتPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریwild type - نوع وحشیluteinizing hormone - هورمون جسم زردVisual processing - پردازش تصویریMeso Scale Discovery - کشف مقیاس Mesohuman chorionic gonadotropin - گونادوتروپین کوریونی انسانLuteinizing hormone receptor - گیرنده هورمون لوتئینیزاسیون
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Luteinizing hormone (LH), produced in the anterior pituitary, has been detected in cadaver eyes and LH receptors (LHRs) have been identified in the retina, with the highest density in cone photoreceptors. Our aim was to confirm the presence of LH in the living, human eye as well as to examine the potential impact of a reduction in LHR signaling on visual processing. Vitreous samples were collected from 40 patients (23 diabetics, 17 non-diabetics) who were undergoing vitrectomies for various indications. LH concentration was quantified in each sample via an electro-chemiluminescence immunoassay and Meso Scale Discovery platform and normalized to total protein. In addition, full-field electroretinography (ERG) was performed on 11 adult LHR knockout heterozygous mice (B6;129X1-Lhcgrtm1Zmlei/J) and 11 wild types using the Celeris-Diagnosys system. The median LH values (pg/mg total protein) for non-diabetics, diabetics without proliferative diabetic retinopathy (PDR) and diabetics with PDR were 40.7, 41.9 and 167.8 respectively. LH levels were significantly higher in diabetics with PDR. In our ERG investigation, heterozygous LHRKOs were found to have significantly reduced amplitudes of a-wave and b-waves at high stimulus intensities with no significant change in a-wave or b-wave amplitudes at lower intensities; this is consistent with a selective impairment of cone-mediated responses. Our findings confirm LH is present in the adult human eye. Our findings also suggest that a reduction in LH receptor signaling negatively impacts visual processing of the cone photoreceptors. Overall, our study results support the theory that LH likely plays a physiologic role in the eye.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 385, 10 August 2018, Pages 1-10
Journal: Neuroscience - Volume 385, 10 August 2018, Pages 1-10
نویسندگان
Tammy Z. Movsas, Kwoon Y. Wong, Michael D. Ober, Robert Sigler, Zhenmin M. Lei, Arivalagan Muthusamy,