کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8840727 | 1614695 | 2018 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A New Micro-holder Device for Local Drug Delivery during In Vivo Whole-cell Recordings
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کلمات کلیدی
TTXLFPBicucullineStriatum - استریاتومtetrodotoxin - تترو دوتوکسین BMI - شاخص توده بدنیNeuropharmacology - عصب شناسیCortex - قشرprimary somatosensory cortex - قشر اسموتیسنسوری اولیهMotor cortex - قشر حرکتیbicuculline methiodide - متیکویدید bicucullineSlow oscillations - نوسانات آهستهlocal field potential - پتانسیل منطقه ای
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Focal administration of pharmacological agents during in vivo recordings is a useful technique to study the functional properties of neural microcircuits. However, the lack of visual control makes this task difficult and inaccurate, especially when targeting small and deep regions where spillover to neighboring regions is likely to occur. An additional problem with recording stability arises when combining focal drug administration with in vivo intracellular recordings, which are highly sensitive to mechanical vibrations. To address these technical issues, we designed a micro-holder that enables accurate local application of pharmacological agents during in vivo whole-cell recordings. The holder couples the recording and drug delivery pipettes with adjustable distance between the respective tips adapted to the experimental needs. To test the efficacy of the micro-holder we first performed whole-cell recordings in mouse primary somatosensory cortex (S1) with simultaneous extracellular recordings in S1 and motor cortex (M1), before and after local application of bicuculline methiodide (BMI 200â¯ÂµM). The blockade of synaptic inhibition resulted in increased amplitudes and rising slopes of “Up states”, and shortening of their duration. We then checked the usability of the micro-holder in a deeper brain structure, the striatum. We applied tetrodotoxin (TTX 10â¯ÂµM) during whole-cell recordings in the striatum, while simultaneously obtaining extracellular recordings in S1 and M1. The focal application of TTX in the striatum blocked Up states in the recorded striatal neurons, without affecting the cortical activity. We also describe two different approaches for precisely releasing the drugs without unwanted leakage along the pipette approach trajectory.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 381, 15 June 2018, Pages 115-123
Journal: Neuroscience - Volume 381, 15 June 2018, Pages 115-123
نویسندگان
MarÃa Sáez, Maya Ketzef, Javier Alegre-Cortés, Ramón Reig, Gilad Silberberg,