کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8841972 1615037 2018 29 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Diabetes aggravates decreases in hippocalcin and parvalbumin expression in focal cerebral ischemia
ترجمه فارسی عنوان
دیابت باعث کاهش بیان هیپوکالسین و پارافبومین در ایسکمی کانونی مغزی می شود
کلمات کلیدی
دیابت، ایسکمی مرکزی مغزی هیپوکاکین، پارافوبومین،
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی
Hyperglycemia is a major risk factor for stroke and increases brain damage during ischemic stroke. Hyperglycemia increases the intracellular calcium concentration after ischemic injury, thereby triggering neuronal cell death. Calcium binding proteins, including hippocalcin and parvalbumin, are critical regulators of intracellular calcium levels. This study aimed to investigate whether hyperglycemic conditions affect hippocalcin and parvalbumin expression during ischemic brain injury. Male adult rats were treated intraperitoneally with streptozotocin (40 mg/kg) to induce hyperglycemia. Four weeks later, cerebral ischemic injury was induced via surgical middle cerebral artery occlusion (MCAO). Cerebral cortex samples were collected 24 h after MCAO. A proteomic approach showed that the protein levels of hippocalcin and parvalbumin were significantly decreased in streptozotocin-treated animals with MCAO injury compared to streptozotocin-treated animals and animals that underwent MCAO alone. Reverse transcription-PCR and Western blot analyses clearly confirmed the decreased levels of these proteins. These decreases indicate dysregulation of the intracellular calcium balance and induction of cell death. Thus, these results suggest that significantly decreased levels of hippocalcin and parvalbumin exacerbate neuronal cell death in diabetic animals with ischemic brain injury.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 662, 1 January 2018, Pages 189-194
نویسندگان
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