کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8865 608 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new approach to biofunctionalisation and micropatterning of multi-well plates
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
A new approach to biofunctionalisation and micropatterning of multi-well plates
چکیده انگلیسی

Biomolecule attachment and lateral micropatterning of biomolecular layers are essential techniques to provide in advanced biochemical and cell culture assays. For that purpose, we introduced a versatile, simple and robust method to functionalise standard polystyrene well plates. Free amino groups were generated on the polystyrene surface by low pressure ammonia plasma treatment. Subsequently, thin films of different maleic anhydride copolymers were covalently attached to the surfaces. The distinct physicochemical properties of the coupled maleic anhydride copolymers provided a broad range of possible attachment schemes of proteins and polysaccharides. Micrometer-sized lateral patterns of these functional coatings were created by plasma etching through silicon masks and subsequent chemical conversion of the etched areas using poly(ethylene glycol). The approach facilitates a wide variety of cell culture experiments allowing a combination of biomolecule coupling and micropatterning within the multi-well plate technology.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 31, Issue 33, November 2010, Pages 8802–8809
نویسندگان
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