کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8875872 | 1623705 | 2019 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MicroRNA-34c regulates porcine granulosa cell function by targeting forkhead box O3a
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
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چکیده انگلیسی
Granulosa cells (GCs) are somatic cells of ovary, the behaviors of GCs are important for ovarian function. MicroRNAs (miRNAs) are a class of endogenous 18-24 nucleotide (nt) non-coding RNAs, some of which have been shown to be important regulators of GCs function. miR-34c involved in the regulation of various biological processes and was identified to be a pro-apoptotic and anti-proliferative factor in many cell types. However, the roles of miR-34c in GCs function remain unknown. In this study, we used Annexin V-FITC and EdU assays to demonstrate that miR-34c exerted pro-apoptotic and anti-proliferative effects in porcine GCs. Dual-luciferase reporter assays, quantitative real-time PCR (qRT-PCR) and Western blotting identified Forkhead box O3a (FoxO3a) as a direct target gene of miR-34c. The overexpression of FoxO3a rescued the phenotypic change caused by miR-34c in porcine GCs. In conclusion, miR-34c regulate the function of porcine GCs by targeting FoxO3a.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Integrative Agriculture - Volume 16, Issue 9, September 2017, Pages 2019-2028
Journal: Journal of Integrative Agriculture - Volume 16, Issue 9, September 2017, Pages 2019-2028
نویسندگان
Yuan XU, Ai-ling ZHANG, Zhe ZHANG, Xiao-long YUAN, Zan-mou CHEN, Hao ZHANG, Jia-qi LI,