کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8883762 | 1625906 | 2018 | 38 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Toxicokinetic and toxicodynamic studies of carbaryl alone or in binary mixtures with azinphos methyl in the freshwater gastropod Planorbarius corneus
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کلمات کلیدی
p-NPBp-NPACyPB-esterasesBCFAcSChCarboxylesterasesDTNBCPFAZMPlanorbarius corneusMixtures - مخلوط هاOPs - OP هاp-nitrophenyl butyrate - p-nitrofenyl butyratep-Nitrophenyl acetate - p-نیترفنیل استاتAChE - آهیAzinphos-methyl - آینفوس متیلAcetylthiocholine iodide - اتیل تیوکولین یدیدOrganophosphates - ارگانوفسفاتAcetylcholinesterase - استیل کولین استرازCES - اینToxicokinetics - تاکسیکوسینتیک، تاکسیکوکینتیکCytochrome P450 - سیتوکروم پی۴۵۰bioconcentration factor - فاکتور زیستی کنسانترهCarbaryl - کاربیریلChlorpyrifos - کلرپیریفوسCHE - کهCholinesterase - کولین استراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Carbamate insecticides such as carbaryl and organophosphates such as azinphos-methyl share the ability to inhibit the activity of B-esterases. This study aimed to (1) assess the inhibitory effects of carbaryl on B-esterase activity in soft tissues and hemolymph of Planorbarius corneus; (2) establish whether binary mixtures of carbaryl and azinphos-methyl depart or not from a model of concentration addition on the inhibition of cholinesterase activity; (3) determine the bioconcentration and elimination of the pesticides. The results showed that exposure of gastropods to increasing concentrations of carbaryl (0.1-5â¯mgâ¯Lâ1) for 48â¯h inhibited cholinesterase activity in a concentration-dependent manner, with an EC50 of 1.4â¯Â±â¯0.3â¯mgâ¯Lâ1 and 1.2â¯Â±â¯0.1â¯mgâ¯Lâ1 for soft tissue and hemolymph, respectively. Carboxylesterase activity, measured with the substrates p-nitrophenyl butyrate and p-nitrophenyl acetate, was between 2.3 and 25 times more sensitive to carbaryl inhibition than cholinesterase activity. Binary mixtures corresponding to 0.5 EC50 carbarylâ¯+â¯0.5 EC50 azinphos-methyl and 0.75 EC50 carbarylâ¯+â¯0.75 EC50 azinphos-methyl produced inhibitions of cholinesterase activity similar to those of individual pesticides, following a model of concentration addition. Bioconcentration was analyzed using a one-compartment model. The absorption kinetics (k1) for both pesticides alone (1.4â¯mgâ¯Lâ1 of carbaryl or 1.8â¯mgâ¯Lâ1 of azinphos-methyl) or mixed (1.4â¯mgâ¯Lâ1 of carbarylâ¯+â¯1.8â¯mgâ¯Lâ1 of azinphos-methyl) were similar. The elimination kinetics ratio (k2) estimated for the pesticides alone or in the mixtures showed that carbaryl was eliminated 3.5 times faster than azinphos-methyl. These results suggest that exposure of Planorbarius corneus to binary mixtures of carbaryl and azinphos-methyl for 48â¯h follow a concentration addition model on inhibition of cholinesterase activity and that the pesticide mixtures do not change the toxicokinetic parameters of the parent compounds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 199, June 2018, Pages 276-284
Journal: Aquatic Toxicology - Volume 199, June 2018, Pages 276-284
نویسندگان
Luis Claudio Cacciatore, Noemà Rosario Verrengia Guerrero, Adriana Cristina Cochón,