کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8917873 | 1642791 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reproducibility assessment for a broad spectrum drug screening method from urine using liquid chromatography time-of-flight mass spectrometry
ترجمه فارسی عنوان
ارزیابی بازتوزیع برای روش غربالگری داروهای وسیع از ادرار با استفاده از طیف سنجی جرمی کروماتوگرافی مایع
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کلمات کلیدی
غربالگری وسیع طیف، طیف سنجی جرمی، کیفیت بالا، سم شناسی،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
طیف سنجی
چکیده انگلیسی
During the reproducibility validation for a time-of-flight (TOF) high-resolution mass spectrometry (HRMS) method set up to detect 61 drugs of abuse commonly encountered in the toxicology laboratory, it was noticed that, a number of compounds were not identified correctly during the between run analysis; the most difficult compounds to identify were norpropoxyphene, morphine, norbuprenorphine, nortriptyline, EDDP and tramadol. In subsequent patient comparison studies, screening a panel of 338 analytes, the TOF-HRMS method correctly identified 211 analytes over two runs, but did not identify 127. A total of 11 false positive results were identified by manual review of the data to be the result of confirmation ion signal-to-noise ratio(s)â¯<â¯3, although one false positive that was difficult to resolve (i.e., identification of maprotiline as amitriptyline) was due to similar fragment ions and retention times. The TOF-HRMS method showed reasonable agreement with LC-MS/MS results, but there were a number of discrepant results. Additionally, the TOF-HRMS did detect five compounds missed by the LC-MS/MS methods. This extensive validation effort highlights the difficulty of analysis for certain compounds that are likely to require additional follow up prior to reporting a positive result, especially at low and high concentrations, regardless of the type of instrumentation involved.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Mass Spectrometry - Volume 8, April 2018, Pages 1-7
Journal: Clinical Mass Spectrometry - Volume 8, April 2018, Pages 1-7
نویسندگان
Imir G. Metushi, Michael R. Wakefield, Kara Lynch, Judy Stone, Robert L. Fitzgerald,