کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8921803 1643387 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pegylated doxorubicin gold complex: From nanovector to potential intercalant agent for biosensor applications
ترجمه فارسی عنوان
مجتمع طلای دوکسوروبیسین: از نانوساختار به عامل بالقوه متقابل برای کاربردهای بیوسنسور
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی
We report an original approach to synthesize hybrid gold nanostructures in which doxorubicin (DOX), mixed to Poliethylenglycole diacid (PEG-COOH) led to original hybrid gold nanovector (DOX IN PEG AuNPs). In this work, we investigate the ability of DOX IN PEG-AuNPs to detect the amplification of the hybridization process by a sensitive Quartz crystal Microbalance with dissipation (QCM-D) by intercalation process. The sensing layer was carried out by self-assembled monolayer of β mercaptoethylamine (cysteamine) on gold-coated quartz crystal sensor composed by a rigid homobifunctional cross-linker 1,4 phenilenediisothiocyanate (PDITC) linked covalently with amino-probe oligonucleotides. By QCM characterization in the range from 8 µM to 20 nM, we demonstrate high specificity of DOX IN PEG-AuNPs-DNA with a limit of detection (LOD) of 9 nM. This result is very promising for development of sensitive and effective nanoparticle-based biosensor for quantifying small biomolecules concentration in physiological liquids. These results open a possibility to realize a new class of nanovector which will be tailored for different biomedical application, such as imaging, targeting and drugs delivery.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Frontiers in Laboratory Medicine - Volume 1, Issue 3, September 2017, Pages 114-121
نویسندگان
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