کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8932 610 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of electrical stimulation on the differentiation of hESCs adhered onto fibronectin-coated gold nanoparticles
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
The effect of electrical stimulation on the differentiation of hESCs adhered onto fibronectin-coated gold nanoparticles
چکیده انگلیسی

To encourage stem cell differentiation, gold nanoparticles (20 nm) were used to deliver electrical stimulation to human embryonic stem cells (hESCs) in vitro. Nano-structured gold nanoparticles were designed by coating the surface of culture dishes with gold nanoparticles using a layer-by-layer (LBL) system. In this method, gold nanoparticles were continuously coated onto dishes by SEM analysis. Evaluation of gene modified hESCs that were subsequently attached onto fibronectin-coated gold nanoparticles revealed that the un-differentiation marker, Oct-4, was no longer present following electrical stimulation. In addition, the osteogenic markers of collagen type I and Cbfa1 increased in response to electrical stimulation, while those of hESCs were not observed without electrical stimulation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 30, Issue 29, October 2009, Pages 5631–5638
نویسندگان
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