کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8943716 | 1645202 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Covalent immobilization of laccase by one pot three component reaction and its application in the decolorization of textile dyes
ترجمه فارسی عنوان
بی تحرک کووالانتی لاکتوز توسط یک واکنش سه جزء پخته و کاربرد آن در رنگ آمیزی رنگ های نساجی
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
In present study, laccase from Myceliophthora thermophila, was immobilized on epoxy-functionalized silica via one-pot three component reaction as a novel and efficient method for immobilization. The results revealed immobilization of 50â¯mg of M. thermophila laccase on 1â¯g of the supports in presence of cyclohexyl isocyanide after 12â¯h of incubation. The immobilized enzyme exhibited notable activity (50â¯U/g) with improved stability toward pH, temperature and organic solvents. Laccase derivative was used for decolorization of five textile dyes (acid orange 156, acid red 52, coomassie brilliant blue, methyl violet, malachite green) with or without the redox mediators such as 1-hydroxybenzotriazole (HBT), catechol, syringaldehyde and N-hydroxyphthalimide (HPT). The results showed that laccase/mediator systems were effective biocatalysts for the treatment of textile dyes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 120, Part A, December 2018, Pages 144-151
Journal: International Journal of Biological Macromolecules - Volume 120, Part A, December 2018, Pages 144-151
نویسندگان
Fatemeh Salami, Zohreh Habibi, Maryam Yousefi, Mehdi Mohammadi,