کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8959624 | 1646335 | 2018 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Generation of isogenic single and multiplex gene knockout mice by base editing-induced STOP
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Generation of isogenic single and multiplex gene knockout mice by base editing-induced STOP Generation of isogenic single and multiplex gene knockout mice by base editing-induced STOP](/preview/png/8959624.png)
چکیده انگلیسی
Although CRISPR/Cas9 has been widely used to generate knockout mice, two major limitations remain: the founders usually carry a mixture of genotypes, and mosaicism harboring multiple genotypes. Therefore, it takes a long time to get homozygous mutants. Recently developed base editing (BE) system, which introduces C-to-T conversion without double strand DNA cleavage, has been used to introduce artificial stop codons (i-STOP) to prematurely terminate translation, providing a cleaner strategy for genome engineering. Using this strategy, we generated CD160 KO and VISTA/CD160 double KO mice by microinjection of a single sgRNA targeting CD160 and a mixture of sgRNAs targeting VISTA and CD160, respectively. The BE system induced STOP efficiently in mouse embryos and consequently in founder mice without detectable off-target. Most interestingly, the majority of the mutants harbor same genetic modifications, indicating we generated isogenic single and multiplex gene mutant mice by BE-induced STOP. We also obtained homozygous mutant mouse in F1 mice, demonstrating the accelerated strategy in generating animal models.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Science Bulletin - Volume 63, Issue 17, 15 September 2018, Pages 1101-1107
Journal: Science Bulletin - Volume 63, Issue 17, 15 September 2018, Pages 1101-1107
نویسندگان
Guang Yang, Tianyu Zhu, Zongyang Lu, Guanglei Li, Hao Zhang, Songjie Feng, Yajing Liu, Jianan Li, Yu Zhang, Jia Chen, Xuejiang Guo, Xingxu Huang,