کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8969063 1551714 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new site-specific integration system for mycobacteria
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
A new site-specific integration system for mycobacteria
چکیده انگلیسی
Site-specific integration into the mycobacterial chromosome can produce stable transformants useful for understanding pathogenesis. However, gene expression can be problematic at certain sites of integration. We have used the Streptomyces φC31 integration system to integrate vector DNA into Mycobacterium smegmatis, M. bovis BCG, and M. tuberculosis through site-specific recombination. A single dominant insertion site was found in M. smegmatis, as previously reported. Three different insertion sites were found in M. bovis BCG. In M. smegmatis, integrated vectors appear to be far more stable than episomal plasmids during unselected passage in vitro, although excision products are detectable. Plasmids based on the φC31 integration system could make useful tools for the study of mycobacterial genetics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tuberculosis - Volume 85, Issues 5–6, September–November 2005, Pages 317-323
نویسندگان
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