کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8978264 | 1106591 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Participation of serum and membrane lectins on the oxidative burst regulation in Macrobrachium rosenbergii hemocytes
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کلمات کلیدی
GlcNAcN-acetyl-neuraminic acidVHSImmune-regulationGalNAcphorbol 12-myristate 13-acetateMRLNBTNeu5AcN-acetyl-d-glucosaminePMA - LDC هاN-Acetyl-D-Galactosamine - N-استیل D-گالاکتوزامینnitroblue tetrazolium - tetrazolium nitroblueFreshwater prawn - آجیل آب شیرینlectins - لکتین هاOxidative burst - پشت سر گذاشتن اکسیداتیوglycoproteins - گلیکوپروتئین ها
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Using a spectrophotometric NBT reduction assay and phagocytosis, we identified that production of superoxide anions and phagocytic activity of hemocytes from Macrobrachium rosenbergii were significantly higher in the presence of rat, rabbit, and chicken erythrocytes than with human, pig, or horse erythrocytes. Hemocytes stimulated with MrL, MrLMab, or PMA increased 4.7, 5.1, and 6.1 fold, respectively, the oxidative response as compared to non-stimulated hemocytes. MrLMab together with MrL increased 5.7 fold the oxidative capacity of hemocytes as compared to non-stimulated cells. These effects were inhibited with 100 mM GalNAc, GlcNAc, or Neu5Ac and 0.2 μM of sialylated submaxillary gland mucin and fetuin. Piroxicam inhibited (P<0.05) the production of O2â induced by MrL, whereas iodoacetamide inhibited the effect of MrLMAb (P<0.05) in a dose-dependent manner. Our results suggest that MrLMab might activate the oxidative burst through the metabolism of glucose as opposed to MrL which utilizes NADPH-independent mechanisms, very probably through pro-inflammatory metabolites.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 29, Issue 2, 2005, Pages 113-121
Journal: Developmental & Comparative Immunology - Volume 29, Issue 2, 2005, Pages 113-121
نویسندگان
Claudia Sierra, Ricardo Lascurain, Ali Pereyra, Jorge Guevara, Gisela MartÃnez, Concepción Agundis, Edgar Zenteno, Lorena Vázquez,