کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8987665 | 1555131 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential expression and secretion of bovine serum amyloid A3 (SAA3) by mammary epithelial cells stimulated with prolactin or lipopolysaccharide
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کلمات کلیدی
RT-PCRPRLSAASAA1serum amyloid A1SAA3serum amyloid A3cDNA - cDNAComplementary DNA - DNA تکمیلیserum amyloid A - سرم آمیلوئید AMammary gland - غده شیریlipopolysaccharide - لیپوپلی ساکاریدMastitis - ماستیتreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسProlactin - پرولاکتین Colostrum - یخ زده
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Serum amyloid A (SAA) proteins were originally identified as prominent acute phase serum reactants synthesized predominately by hepatocytes in response to infection, inflammation and trauma. In this study, we report the differential expression and secretion of serum amyloid A3 (SAA3) by bovine mammary epithelial cells following stimulation with either prolactin (PRL) or lipopolysaccharide (LPS). Reverse transcription-polymerase chain reaction analysis of PRL or LPS induced bovine mammary epithelial cells resulted in the detection of only the mammary-derived Saa3 (M-Saa3) transcript. Two-dimensional immunoblot analyses of colostrum and milk from healthy cows, as well as conditioned medium from PRL or LPS stimulated bovine mammary epithelial cells confirmed the differential production and secretion of M-SAA3 while other SAA isoforms were not detected. These data indicate that the bovine Saa3 gene is regulated differently from the other Saa genes with regard to the site of and stimulus for expression, suggesting an important tissue-specific function for bovine M-SAA3 during lactation and mammary infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 107, Issues 3â4, 15 September 2005, Pages 255-264
Journal: Veterinary Immunology and Immunopathology - Volume 107, Issues 3â4, 15 September 2005, Pages 255-264
نویسندگان
Marilynn A. Larson, Annika Weber, Allen T. Weber, Thomas L. McDonald,