کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8987848 | 1555129 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro effect of recombinant human granulocyte colony-stimulating factor on canine neutrophil apoptosis
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کلمات کلیدی
GAPDHPMNsDHRCHXphorbol 12-myristate 13-acetaterhG-CSFFITCDMSO - DMSOPMA - LDC هاROS - ROSApoptosis - خزان یاختهایdihydrorhodamine 123 - دی هیدرو رودامین 123Dimethyl sulfoxide - دیمتیل سولفواکسیدDog - سگcycloheximide - سیکلوهایسیمیدRecombinant human granulocyte colony-stimulating factor - فاکتور تحریک کننده گرانولوسیت انسانی انسانیG-CSF - فاکتور محرک کُلونی گرانولوسیتfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتpolymorphonuclear leukocytes - لکوسیت های پلی مورفونیک هسته ایneutrophil - نوتروفیلpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازPropidium iodide - پروتئین یدیدReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Apoptosis is essential in eliminating neutrophils (polymorphonuclear leukocytes: PMNs) in animals. The suppression of PMN apoptosis is believed to be beneficial in eradicating pathogens and is implicated in the pathogenesis of human inflammatory diseases. In the present study, canine PMNs were stimulated with recombinant human granulocyte colony-stimulating factor (rhG-CSF) to investigate the in vitro effect on the apoptosis of canine PMNs. Apoptotic cell rates were assessed by flow cytometry in relation to the ability of PMNs to produce reactive oxygen species (ROS). Canine PMN apoptosis was markedly suppressed by rhG-CSF treatment, in association with the retention of the PMN ability to produce ROS. The addition of cycloheximide abolished this suppression by rhG-CSF. Moreover, canine PMNs, which were stimulated by rhG-CSF, expressed high levels of anti-apoptotic mcl-1 gene mRNA, as quantified by real-time polymerase chain reaction method. The results suggest that PMNs, stimulated by G-CSF, could work effectively over a longer period to eliminate pathogens, and that the prolongation of the PMN life-span might occasionally aggravate tissue injuries in dogs. In addition, the suppression of PMN apoptosis seems to be mediated by the induction of anti-apoptotic mcl-1 gene expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 108, Issues 3â4, 15 December 2005, Pages 307-314
Journal: Veterinary Immunology and Immunopathology - Volume 108, Issues 3â4, 15 December 2005, Pages 307-314
نویسندگان
Keisuke Oguma, Junichi Sano, Rui Kano, Toshihiro Watari, Tadaaki Moritomo, Atsuhiko Hasegawa,